cell signal 2013  (Cell Signaling Technology Inc)

Journal: Physiological reports

Article Title: Mitochondrial A-kinase anchoring proteins in cardiac ventricular myocytes.

doi: 10.14814/phy2.15015

Figure Lengend Snippet: FIGURE 2 Expression levels of mitochondria-associated AKAPs in ARVM. (a) Ponceau S (Pon-S) staining as a loading control. Lanes represent total cell lysate (T), the cytosolic fraction (C), and the mitochondria enriched fraction (M) from ARVMs. (b) Tubulin was used as a marker for the cytosolic fraction and TOMM20 as the specific mitochondrial marker. (c) PVDF membrane was probed for indicated AKAPs. Molecular weight markers are placed adjacent to the blots. n ≥ 3 independent extractions

Article Snippet: Tubulin and TOMM20 were used as markers for the cytosolic and mitochondrial cell fractions, respectively (Figure T A B L E 1 Information on antibodies used for biochemical studies Antibody Manufacturer/Catalog number WB dilution IF dilution References Primary antibodies ACBD3 Santa Cruz Biotech sc−101277 1:500 1:100 Teoule et al., 2013; Greninger et al., 2012; Smola et al., 2020) D- AKAP1 MyBioSource MBS8243156 1:800 1:100 Fernández- Araujo et al., 2014) D- AKAP2 Abnova H00011216- M04 1:100 Eggers et al., 2009) D- AKAP2 Santa Cruz Biotech sc−98755 1:500 OPA1 Santa Cruz Biotech sc−393296 1:500 1:100 Huang et al., 2018; Kim et al., 2021; Koo & Kang, 2019; Xu et al., 2019; Li et al., 2019) Rab32 Santa Cruz Biotech sc−390178 1:500 Kalogeropulou et al., 2020; Hu et al., 2019; Balci et al., 2020) WAVE−1 Santa Cruz Biotech sc−271507 1:500 Moore et al., 2021; Vassilev et al., 2017) SPHKAP Invitrogen PA5- 27581 1:800 PKA RI- α/β Santa Cruz Biotech sc−271125 1:500 1:50 Chen et al., 2017; Chiba et al., 2011) PKA RII- α Santa Cruz Biotech sc−137220 1:500 1:50 Muñoz- Llancao et al., 2017; Clister et al., 2019) PKA RII- β Santa Cruz Biotech sc−376778 1:500 1:50 Just- Borràs et al., 2020; Garcia et al., 2019; Chen et al., 2019) PKA Cat- α/β/γ Santa Cruz Biotech sc−365615 1:500 Tibenska et al., 2020; Pérez- Gómez & Tasker, 2013; Seward et al., 2013) TOMM20 Cell Signaling Technology 42406 1:1000 1:100 Nie et al., 2020; Liu et al., 2019) TOMM20 Abcam ab56783 1:100 Casey et al., 2000; Tavalin et al., 2002) SERCA2 Invitrogen MA3- 919 1:1000 Rudokas et al., 2021; HadipourLakmehsari et al., 2019) Na,K- ATPase α1 Cell Signaling Technology 3010S 1:1000 Lou et al., 2017; Lichý et al., 2019) Tubulin Sigma Aldrich T5168 1:15000 Secondary antibodies Goat anti- mouse Alexa Fluor 568 Invitrogen A11004 1:1000 Goat anti- rabbit Alexa Fluor 647 Invitrogen A32733 1:1000 Peroxidase- AffiniPure Goat Anti- Mouse IgG Jackson ImmunoResearch 115– 035– 003 1:5000 Peroxidase AffiniPure Goat Anti- Rabbit IgG Jackson ImmunoResearch 111– 035– 003 1:5000 2051817x, 2021, 17, D ow nloaded from https://physoc.onlinelibrary.w iley.com /doi/10.14814/phy2.15015, W iley O nline L ibrary on [29/04/2024].

Techniques: Expressing, Staining, Control, Marker, Membrane, Molecular Weight

Journal: Physiological reports

Article Title: Mitochondrial A-kinase anchoring proteins in cardiac ventricular myocytes.

doi: 10.14814/phy2.15015

Figure Lengend Snippet: FIGURE 3 Co-localization of D-AKAP1 and mitochondria in ARVM. Representative confocal images of ARVM showing mitochondrial marker (a) TOMM20 and (b) D-AKAP1. (c) Merged image of TOMM20 (red), D-AKAP1 (green), and DAPI (blue) shows that D-AKAP1 overlaps the mitochondrial marker. PCC = 0.77 ± 0.03; N = 4 independent isolations with n = 6 from each isolation. Magnified images are shown below each corresponding image with the appropriate scale bar

Article Snippet: Tubulin and TOMM20 were used as markers for the cytosolic and mitochondrial cell fractions, respectively (Figure T A B L E 1 Information on antibodies used for biochemical studies Antibody Manufacturer/Catalog number WB dilution IF dilution References Primary antibodies ACBD3 Santa Cruz Biotech sc−101277 1:500 1:100 Teoule et al., 2013; Greninger et al., 2012; Smola et al., 2020) D- AKAP1 MyBioSource MBS8243156 1:800 1:100 Fernández- Araujo et al., 2014) D- AKAP2 Abnova H00011216- M04 1:100 Eggers et al., 2009) D- AKAP2 Santa Cruz Biotech sc−98755 1:500 OPA1 Santa Cruz Biotech sc−393296 1:500 1:100 Huang et al., 2018; Kim et al., 2021; Koo & Kang, 2019; Xu et al., 2019; Li et al., 2019) Rab32 Santa Cruz Biotech sc−390178 1:500 Kalogeropulou et al., 2020; Hu et al., 2019; Balci et al., 2020) WAVE−1 Santa Cruz Biotech sc−271507 1:500 Moore et al., 2021; Vassilev et al., 2017) SPHKAP Invitrogen PA5- 27581 1:800 PKA RI- α/β Santa Cruz Biotech sc−271125 1:500 1:50 Chen et al., 2017; Chiba et al., 2011) PKA RII- α Santa Cruz Biotech sc−137220 1:500 1:50 Muñoz- Llancao et al., 2017; Clister et al., 2019) PKA RII- β Santa Cruz Biotech sc−376778 1:500 1:50 Just- Borràs et al., 2020; Garcia et al., 2019; Chen et al., 2019) PKA Cat- α/β/γ Santa Cruz Biotech sc−365615 1:500 Tibenska et al., 2020; Pérez- Gómez & Tasker, 2013; Seward et al., 2013) TOMM20 Cell Signaling Technology 42406 1:1000 1:100 Nie et al., 2020; Liu et al., 2019) TOMM20 Abcam ab56783 1:100 Casey et al., 2000; Tavalin et al., 2002) SERCA2 Invitrogen MA3- 919 1:1000 Rudokas et al., 2021; HadipourLakmehsari et al., 2019) Na,K- ATPase α1 Cell Signaling Technology 3010S 1:1000 Lou et al., 2017; Lichý et al., 2019) Tubulin Sigma Aldrich T5168 1:15000 Secondary antibodies Goat anti- mouse Alexa Fluor 568 Invitrogen A11004 1:1000 Goat anti- rabbit Alexa Fluor 647 Invitrogen A32733 1:1000 Peroxidase- AffiniPure Goat Anti- Mouse IgG Jackson ImmunoResearch 115– 035– 003 1:5000 Peroxidase AffiniPure Goat Anti- Rabbit IgG Jackson ImmunoResearch 111– 035– 003 1:5000 2051817x, 2021, 17, D ow nloaded from https://physoc.onlinelibrary.w iley.com /doi/10.14814/phy2.15015, W iley O nline L ibrary on [29/04/2024].

Techniques: Marker, Isolation

Journal: Physiological reports

Article Title: Mitochondrial A-kinase anchoring proteins in cardiac ventricular myocytes.

doi: 10.14814/phy2.15015

Figure Lengend Snippet: FIGURE 4 Co-localization of D-AKAP2 and mitochondria in ARVM. Confocal images of ARVM showing mitochondrial marker (a) TOMM20 and (b) D-AKAP2, presenting a punctate pattern and overlap with mitochondria. (c) Merged images representing TOMM20 (red), D-AKAP2 (green), and DAPI (blue). PCC = 0.72 ± 0.03; N = 4 independent isolations with n = 6 from each isolation. Magnified images are shown below each corresponding image with the appropriate scale bar

Article Snippet: Tubulin and TOMM20 were used as markers for the cytosolic and mitochondrial cell fractions, respectively (Figure T A B L E 1 Information on antibodies used for biochemical studies Antibody Manufacturer/Catalog number WB dilution IF dilution References Primary antibodies ACBD3 Santa Cruz Biotech sc−101277 1:500 1:100 Teoule et al., 2013; Greninger et al., 2012; Smola et al., 2020) D- AKAP1 MyBioSource MBS8243156 1:800 1:100 Fernández- Araujo et al., 2014) D- AKAP2 Abnova H00011216- M04 1:100 Eggers et al., 2009) D- AKAP2 Santa Cruz Biotech sc−98755 1:500 OPA1 Santa Cruz Biotech sc−393296 1:500 1:100 Huang et al., 2018; Kim et al., 2021; Koo & Kang, 2019; Xu et al., 2019; Li et al., 2019) Rab32 Santa Cruz Biotech sc−390178 1:500 Kalogeropulou et al., 2020; Hu et al., 2019; Balci et al., 2020) WAVE−1 Santa Cruz Biotech sc−271507 1:500 Moore et al., 2021; Vassilev et al., 2017) SPHKAP Invitrogen PA5- 27581 1:800 PKA RI- α/β Santa Cruz Biotech sc−271125 1:500 1:50 Chen et al., 2017; Chiba et al., 2011) PKA RII- α Santa Cruz Biotech sc−137220 1:500 1:50 Muñoz- Llancao et al., 2017; Clister et al., 2019) PKA RII- β Santa Cruz Biotech sc−376778 1:500 1:50 Just- Borràs et al., 2020; Garcia et al., 2019; Chen et al., 2019) PKA Cat- α/β/γ Santa Cruz Biotech sc−365615 1:500 Tibenska et al., 2020; Pérez- Gómez & Tasker, 2013; Seward et al., 2013) TOMM20 Cell Signaling Technology 42406 1:1000 1:100 Nie et al., 2020; Liu et al., 2019) TOMM20 Abcam ab56783 1:100 Casey et al., 2000; Tavalin et al., 2002) SERCA2 Invitrogen MA3- 919 1:1000 Rudokas et al., 2021; HadipourLakmehsari et al., 2019) Na,K- ATPase α1 Cell Signaling Technology 3010S 1:1000 Lou et al., 2017; Lichý et al., 2019) Tubulin Sigma Aldrich T5168 1:15000 Secondary antibodies Goat anti- mouse Alexa Fluor 568 Invitrogen A11004 1:1000 Goat anti- rabbit Alexa Fluor 647 Invitrogen A32733 1:1000 Peroxidase- AffiniPure Goat Anti- Mouse IgG Jackson ImmunoResearch 115– 035– 003 1:5000 Peroxidase AffiniPure Goat Anti- Rabbit IgG Jackson ImmunoResearch 111– 035– 003 1:5000 2051817x, 2021, 17, D ow nloaded from https://physoc.onlinelibrary.w iley.com /doi/10.14814/phy2.15015, W iley O nline L ibrary on [29/04/2024].

Techniques: Marker, Isolation

Journal: Physiological reports

Article Title: Mitochondrial A-kinase anchoring proteins in cardiac ventricular myocytes.

doi: 10.14814/phy2.15015

Figure Lengend Snippet: FIGURE 5 Co- labeling of ACBD3 with mitochondria in ARVM. Labeling of the mitochondrial marker, (a) TOMM20 and (b) ACBD3 show a similar pattern of immunoreactivity. (c) Merged images of TOMM20 (red), ACBD3 (green), and DAPI (blue) show that ACBD3 is distributed in a diffuse manner generating a PCC of 0.56 ± 0.02; N = 4 independent isolations with n = 6 from each isolation. Magnified images are shown below each corresponding image with the appropriate scale bar

Article Snippet: Tubulin and TOMM20 were used as markers for the cytosolic and mitochondrial cell fractions, respectively (Figure T A B L E 1 Information on antibodies used for biochemical studies Antibody Manufacturer/Catalog number WB dilution IF dilution References Primary antibodies ACBD3 Santa Cruz Biotech sc−101277 1:500 1:100 Teoule et al., 2013; Greninger et al., 2012; Smola et al., 2020) D- AKAP1 MyBioSource MBS8243156 1:800 1:100 Fernández- Araujo et al., 2014) D- AKAP2 Abnova H00011216- M04 1:100 Eggers et al., 2009) D- AKAP2 Santa Cruz Biotech sc−98755 1:500 OPA1 Santa Cruz Biotech sc−393296 1:500 1:100 Huang et al., 2018; Kim et al., 2021; Koo & Kang, 2019; Xu et al., 2019; Li et al., 2019) Rab32 Santa Cruz Biotech sc−390178 1:500 Kalogeropulou et al., 2020; Hu et al., 2019; Balci et al., 2020) WAVE−1 Santa Cruz Biotech sc−271507 1:500 Moore et al., 2021; Vassilev et al., 2017) SPHKAP Invitrogen PA5- 27581 1:800 PKA RI- α/β Santa Cruz Biotech sc−271125 1:500 1:50 Chen et al., 2017; Chiba et al., 2011) PKA RII- α Santa Cruz Biotech sc−137220 1:500 1:50 Muñoz- Llancao et al., 2017; Clister et al., 2019) PKA RII- β Santa Cruz Biotech sc−376778 1:500 1:50 Just- Borràs et al., 2020; Garcia et al., 2019; Chen et al., 2019) PKA Cat- α/β/γ Santa Cruz Biotech sc−365615 1:500 Tibenska et al., 2020; Pérez- Gómez & Tasker, 2013; Seward et al., 2013) TOMM20 Cell Signaling Technology 42406 1:1000 1:100 Nie et al., 2020; Liu et al., 2019) TOMM20 Abcam ab56783 1:100 Casey et al., 2000; Tavalin et al., 2002) SERCA2 Invitrogen MA3- 919 1:1000 Rudokas et al., 2021; HadipourLakmehsari et al., 2019) Na,K- ATPase α1 Cell Signaling Technology 3010S 1:1000 Lou et al., 2017; Lichý et al., 2019) Tubulin Sigma Aldrich T5168 1:15000 Secondary antibodies Goat anti- mouse Alexa Fluor 568 Invitrogen A11004 1:1000 Goat anti- rabbit Alexa Fluor 647 Invitrogen A32733 1:1000 Peroxidase- AffiniPure Goat Anti- Mouse IgG Jackson ImmunoResearch 115– 035– 003 1:5000 Peroxidase AffiniPure Goat Anti- Rabbit IgG Jackson ImmunoResearch 111– 035– 003 1:5000 2051817x, 2021, 17, D ow nloaded from https://physoc.onlinelibrary.w iley.com /doi/10.14814/phy2.15015, W iley O nline L ibrary on [29/04/2024].

Techniques: Labeling, Marker, Isolation

Journal: Physiological reports

Article Title: Mitochondrial A-kinase anchoring proteins in cardiac ventricular myocytes.

doi: 10.14814/phy2.15015

Figure Lengend Snippet: FIGURE 6 Co- labeling of OPA1 with mitochondria in ARVM. Labeling of the mitochondrial marker, (a) TOMM20 and (b) OPA1. (c) Merged images of TOMM20 (red), OPA1 (green), and DAPI (blue). PCC = 0.58 ± 0.03; N = 4 independent isolations with n = 6 from each isolation. Magnified images are shown below each corresponding image with the appropriate scale bar

Article Snippet: Tubulin and TOMM20 were used as markers for the cytosolic and mitochondrial cell fractions, respectively (Figure T A B L E 1 Information on antibodies used for biochemical studies Antibody Manufacturer/Catalog number WB dilution IF dilution References Primary antibodies ACBD3 Santa Cruz Biotech sc−101277 1:500 1:100 Teoule et al., 2013; Greninger et al., 2012; Smola et al., 2020) D- AKAP1 MyBioSource MBS8243156 1:800 1:100 Fernández- Araujo et al., 2014) D- AKAP2 Abnova H00011216- M04 1:100 Eggers et al., 2009) D- AKAP2 Santa Cruz Biotech sc−98755 1:500 OPA1 Santa Cruz Biotech sc−393296 1:500 1:100 Huang et al., 2018; Kim et al., 2021; Koo & Kang, 2019; Xu et al., 2019; Li et al., 2019) Rab32 Santa Cruz Biotech sc−390178 1:500 Kalogeropulou et al., 2020; Hu et al., 2019; Balci et al., 2020) WAVE−1 Santa Cruz Biotech sc−271507 1:500 Moore et al., 2021; Vassilev et al., 2017) SPHKAP Invitrogen PA5- 27581 1:800 PKA RI- α/β Santa Cruz Biotech sc−271125 1:500 1:50 Chen et al., 2017; Chiba et al., 2011) PKA RII- α Santa Cruz Biotech sc−137220 1:500 1:50 Muñoz- Llancao et al., 2017; Clister et al., 2019) PKA RII- β Santa Cruz Biotech sc−376778 1:500 1:50 Just- Borràs et al., 2020; Garcia et al., 2019; Chen et al., 2019) PKA Cat- α/β/γ Santa Cruz Biotech sc−365615 1:500 Tibenska et al., 2020; Pérez- Gómez & Tasker, 2013; Seward et al., 2013) TOMM20 Cell Signaling Technology 42406 1:1000 1:100 Nie et al., 2020; Liu et al., 2019) TOMM20 Abcam ab56783 1:100 Casey et al., 2000; Tavalin et al., 2002) SERCA2 Invitrogen MA3- 919 1:1000 Rudokas et al., 2021; HadipourLakmehsari et al., 2019) Na,K- ATPase α1 Cell Signaling Technology 3010S 1:1000 Lou et al., 2017; Lichý et al., 2019) Tubulin Sigma Aldrich T5168 1:15000 Secondary antibodies Goat anti- mouse Alexa Fluor 568 Invitrogen A11004 1:1000 Goat anti- rabbit Alexa Fluor 647 Invitrogen A32733 1:1000 Peroxidase- AffiniPure Goat Anti- Mouse IgG Jackson ImmunoResearch 115– 035– 003 1:5000 Peroxidase AffiniPure Goat Anti- Rabbit IgG Jackson ImmunoResearch 111– 035– 003 1:5000 2051817x, 2021, 17, D ow nloaded from https://physoc.onlinelibrary.w iley.com /doi/10.14814/phy2.15015, W iley O nline L ibrary on [29/04/2024].

Techniques: Labeling, Marker, Isolation

Journal: Physiological reports

Article Title: Mitochondrial A-kinase anchoring proteins in cardiac ventricular myocytes.

doi: 10.14814/phy2.15015

Figure Lengend Snippet: FIGURE 8 PKA RI subunit and mitochondria co-labeling. Representative confocal images of ARVM showing mitochondrial marker (a) TOMM20 and (b) PKA RI regulatory subunit. (c) Composite image showing strong overlap of TOMM20 (red) and PKA RI subunit (green) in the subsarcolemmal mitochondria. PKA RI antibody is also seen to label transverse striations. PCC = 0.69 ± 0.02, MOC = 0.80 ± 0.03; N = 4 independent isolations with n = 6 from each isolation. Magnified images are shown below each corresponding image with the appropriate scale bar

Article Snippet: Tubulin and TOMM20 were used as markers for the cytosolic and mitochondrial cell fractions, respectively (Figure T A B L E 1 Information on antibodies used for biochemical studies Antibody Manufacturer/Catalog number WB dilution IF dilution References Primary antibodies ACBD3 Santa Cruz Biotech sc−101277 1:500 1:100 Teoule et al., 2013; Greninger et al., 2012; Smola et al., 2020) D- AKAP1 MyBioSource MBS8243156 1:800 1:100 Fernández- Araujo et al., 2014) D- AKAP2 Abnova H00011216- M04 1:100 Eggers et al., 2009) D- AKAP2 Santa Cruz Biotech sc−98755 1:500 OPA1 Santa Cruz Biotech sc−393296 1:500 1:100 Huang et al., 2018; Kim et al., 2021; Koo & Kang, 2019; Xu et al., 2019; Li et al., 2019) Rab32 Santa Cruz Biotech sc−390178 1:500 Kalogeropulou et al., 2020; Hu et al., 2019; Balci et al., 2020) WAVE−1 Santa Cruz Biotech sc−271507 1:500 Moore et al., 2021; Vassilev et al., 2017) SPHKAP Invitrogen PA5- 27581 1:800 PKA RI- α/β Santa Cruz Biotech sc−271125 1:500 1:50 Chen et al., 2017; Chiba et al., 2011) PKA RII- α Santa Cruz Biotech sc−137220 1:500 1:50 Muñoz- Llancao et al., 2017; Clister et al., 2019) PKA RII- β Santa Cruz Biotech sc−376778 1:500 1:50 Just- Borràs et al., 2020; Garcia et al., 2019; Chen et al., 2019) PKA Cat- α/β/γ Santa Cruz Biotech sc−365615 1:500 Tibenska et al., 2020; Pérez- Gómez & Tasker, 2013; Seward et al., 2013) TOMM20 Cell Signaling Technology 42406 1:1000 1:100 Nie et al., 2020; Liu et al., 2019) TOMM20 Abcam ab56783 1:100 Casey et al., 2000; Tavalin et al., 2002) SERCA2 Invitrogen MA3- 919 1:1000 Rudokas et al., 2021; HadipourLakmehsari et al., 2019) Na,K- ATPase α1 Cell Signaling Technology 3010S 1:1000 Lou et al., 2017; Lichý et al., 2019) Tubulin Sigma Aldrich T5168 1:15000 Secondary antibodies Goat anti- mouse Alexa Fluor 568 Invitrogen A11004 1:1000 Goat anti- rabbit Alexa Fluor 647 Invitrogen A32733 1:1000 Peroxidase- AffiniPure Goat Anti- Mouse IgG Jackson ImmunoResearch 115– 035– 003 1:5000 Peroxidase AffiniPure Goat Anti- Rabbit IgG Jackson ImmunoResearch 111– 035– 003 1:5000 2051817x, 2021, 17, D ow nloaded from https://physoc.onlinelibrary.w iley.com /doi/10.14814/phy2.15015, W iley O nline L ibrary on [29/04/2024].

Techniques: Labeling, Marker, Isolation

Journal: Physiological reports

Article Title: Mitochondrial A-kinase anchoring proteins in cardiac ventricular myocytes.

doi: 10.14814/phy2.15015

Figure Lengend Snippet: FIGURE 9 Co-localization of PKA RIIα subunit with mitochondria. Representative confocal images of ARVM showing mitochondrial marker (a) TOMM20 and (b) PKA RII-α subunit. (c) Merged images of TOMM20 (red), PKA RIIα subunit (green), and DAPI (blue). PCC = 0.63 ± 0.02, MOC = 0.80 ± 0.02; N = 4 independent isolations with n = 6 from each isolation. Magnified images are shown below each corresponding image with the appropriate scale bar

Article Snippet: Tubulin and TOMM20 were used as markers for the cytosolic and mitochondrial cell fractions, respectively (Figure T A B L E 1 Information on antibodies used for biochemical studies Antibody Manufacturer/Catalog number WB dilution IF dilution References Primary antibodies ACBD3 Santa Cruz Biotech sc−101277 1:500 1:100 Teoule et al., 2013; Greninger et al., 2012; Smola et al., 2020) D- AKAP1 MyBioSource MBS8243156 1:800 1:100 Fernández- Araujo et al., 2014) D- AKAP2 Abnova H00011216- M04 1:100 Eggers et al., 2009) D- AKAP2 Santa Cruz Biotech sc−98755 1:500 OPA1 Santa Cruz Biotech sc−393296 1:500 1:100 Huang et al., 2018; Kim et al., 2021; Koo & Kang, 2019; Xu et al., 2019; Li et al., 2019) Rab32 Santa Cruz Biotech sc−390178 1:500 Kalogeropulou et al., 2020; Hu et al., 2019; Balci et al., 2020) WAVE−1 Santa Cruz Biotech sc−271507 1:500 Moore et al., 2021; Vassilev et al., 2017) SPHKAP Invitrogen PA5- 27581 1:800 PKA RI- α/β Santa Cruz Biotech sc−271125 1:500 1:50 Chen et al., 2017; Chiba et al., 2011) PKA RII- α Santa Cruz Biotech sc−137220 1:500 1:50 Muñoz- Llancao et al., 2017; Clister et al., 2019) PKA RII- β Santa Cruz Biotech sc−376778 1:500 1:50 Just- Borràs et al., 2020; Garcia et al., 2019; Chen et al., 2019) PKA Cat- α/β/γ Santa Cruz Biotech sc−365615 1:500 Tibenska et al., 2020; Pérez- Gómez & Tasker, 2013; Seward et al., 2013) TOMM20 Cell Signaling Technology 42406 1:1000 1:100 Nie et al., 2020; Liu et al., 2019) TOMM20 Abcam ab56783 1:100 Casey et al., 2000; Tavalin et al., 2002) SERCA2 Invitrogen MA3- 919 1:1000 Rudokas et al., 2021; HadipourLakmehsari et al., 2019) Na,K- ATPase α1 Cell Signaling Technology 3010S 1:1000 Lou et al., 2017; Lichý et al., 2019) Tubulin Sigma Aldrich T5168 1:15000 Secondary antibodies Goat anti- mouse Alexa Fluor 568 Invitrogen A11004 1:1000 Goat anti- rabbit Alexa Fluor 647 Invitrogen A32733 1:1000 Peroxidase- AffiniPure Goat Anti- Mouse IgG Jackson ImmunoResearch 115– 035– 003 1:5000 Peroxidase AffiniPure Goat Anti- Rabbit IgG Jackson ImmunoResearch 111– 035– 003 1:5000 2051817x, 2021, 17, D ow nloaded from https://physoc.onlinelibrary.w iley.com /doi/10.14814/phy2.15015, W iley O nline L ibrary on [29/04/2024].

Techniques: Marker, Isolation

Journal: Physiological reports

Article Title: Mitochondrial A-kinase anchoring proteins in cardiac ventricular myocytes.

doi: 10.14814/phy2.15015

Figure Lengend Snippet: FIGURE 10 Co-localization of PKA RIIβ subunit with mitochondria. Representative confocal images of ARVM showing mitochondrial marker (a) TOMM20 and (b) PKA RII-β subunit. (c) Merged image shows moderate colocalization between TOMM20 (red), PKA RII-β (green), and DAPI (blue). PCC = 0.61 ± 0.02, MOC = 0.73 ± 0.02; N = 4 independent isolations/experiments with n = 6 from each isolation. Magnified images are shown below each corresponding image with the appropriate scale bar

Article Snippet: Tubulin and TOMM20 were used as markers for the cytosolic and mitochondrial cell fractions, respectively (Figure T A B L E 1 Information on antibodies used for biochemical studies Antibody Manufacturer/Catalog number WB dilution IF dilution References Primary antibodies ACBD3 Santa Cruz Biotech sc−101277 1:500 1:100 Teoule et al., 2013; Greninger et al., 2012; Smola et al., 2020) D- AKAP1 MyBioSource MBS8243156 1:800 1:100 Fernández- Araujo et al., 2014) D- AKAP2 Abnova H00011216- M04 1:100 Eggers et al., 2009) D- AKAP2 Santa Cruz Biotech sc−98755 1:500 OPA1 Santa Cruz Biotech sc−393296 1:500 1:100 Huang et al., 2018; Kim et al., 2021; Koo & Kang, 2019; Xu et al., 2019; Li et al., 2019) Rab32 Santa Cruz Biotech sc−390178 1:500 Kalogeropulou et al., 2020; Hu et al., 2019; Balci et al., 2020) WAVE−1 Santa Cruz Biotech sc−271507 1:500 Moore et al., 2021; Vassilev et al., 2017) SPHKAP Invitrogen PA5- 27581 1:800 PKA RI- α/β Santa Cruz Biotech sc−271125 1:500 1:50 Chen et al., 2017; Chiba et al., 2011) PKA RII- α Santa Cruz Biotech sc−137220 1:500 1:50 Muñoz- Llancao et al., 2017; Clister et al., 2019) PKA RII- β Santa Cruz Biotech sc−376778 1:500 1:50 Just- Borràs et al., 2020; Garcia et al., 2019; Chen et al., 2019) PKA Cat- α/β/γ Santa Cruz Biotech sc−365615 1:500 Tibenska et al., 2020; Pérez- Gómez & Tasker, 2013; Seward et al., 2013) TOMM20 Cell Signaling Technology 42406 1:1000 1:100 Nie et al., 2020; Liu et al., 2019) TOMM20 Abcam ab56783 1:100 Casey et al., 2000; Tavalin et al., 2002) SERCA2 Invitrogen MA3- 919 1:1000 Rudokas et al., 2021; HadipourLakmehsari et al., 2019) Na,K- ATPase α1 Cell Signaling Technology 3010S 1:1000 Lou et al., 2017; Lichý et al., 2019) Tubulin Sigma Aldrich T5168 1:15000 Secondary antibodies Goat anti- mouse Alexa Fluor 568 Invitrogen A11004 1:1000 Goat anti- rabbit Alexa Fluor 647 Invitrogen A32733 1:1000 Peroxidase- AffiniPure Goat Anti- Mouse IgG Jackson ImmunoResearch 115– 035– 003 1:5000 Peroxidase AffiniPure Goat Anti- Rabbit IgG Jackson ImmunoResearch 111– 035– 003 1:5000 2051817x, 2021, 17, D ow nloaded from https://physoc.onlinelibrary.w iley.com /doi/10.14814/phy2.15015, W iley O nline L ibrary on [29/04/2024].

Techniques: Marker, Isolation